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KMID : 0370220040480010020
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Yakhak Hoeji
2004 Volume.48 No. 1 p.20 ~ p.26
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Analysis of Genetic Diversity of Bifidobacterium spp. Isolated from Korean Adults Fecal Samples
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배학균/Bae HG
김선옥/박종선/강병용/최성숙/강진양/하남주/Kim SO/Park JS/Kang BY/Choi SS/Kang CY/Ha NJ
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Abstract
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Twelve strains of Bifidobacterium spp. Were isolated from the feces of healthy Korean 20~30 years. The identification of genera from isolates were performed by the micr oscopic observation and fructose-6-phosphate phosphoketolase (F6PPK) activity, which is the key enzyme to distinguish the Bifidobacterium spp. From other anaerobic bacteria. To determine the antibacterial resistance patterns, minimum inhibitory concentration (MIC) of several antibiotics (including antituberculosis agents) was determined. Five of the isolates showed the high degree of resistance to vancomycin. To investigate the genetic diversity between isolates and type strain of Bifidobacterium spp. From KCTC, we performed the RAPD-fingerprinting. Using a total set of four primers, it is possible to distinguish the isolates and Bifidobacterium spp. From KCTC. Thus, Bifidobacterium strains isolated from our samples may be a new species or strains of Bifidobacterium genera, and have the potential as a probiotics.
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KEYWORD
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